Reducing the Risks of Environmental Pollution by Agents of Biological Origin

L. R. VALIULLIN; R. S. MUKHAMMADIEV; A. S. SOLOVYOVA; E. V. SKVORTSOV; Rin. S. MUKHAMMADIEV; D. A. VALIULLINA; N. R. KASANOVA

doi:10.14505//jemt.v11.3(43).07

L. R. VALIULLIN Federal Center for Toxicological, Radiation and Biological Safety All Russian Research Institute of Phytopathology, Russian Federation
R. S. MUKHAMMADIEV Federal Center for Toxicological, Radiation and Biological Safety, Russian Federation
A. S. SOLOVYOVA Federal Center for Toxicological, Radiation and Biological Safety, Russian Federation
E. V. SKVORTSOV Federal Center for Toxicological, Radiation and Biological Safety, Russian Federation
Rin. S. MUKHAMMADIEV Federal Center for Toxicological, Radiation and Biological Safety, Russian Federation
D. A. VALIULLINA N. E. Bauman Kazan State Academy of Veterinary Medicine, Russian Federation
N. R. KASANOVA N. E. Bauman Kazan State Academy of Veterinary Medicine, Кazan, Russian Federation

DOI: https://doi.org/10.14505//jemt.v11.3(43).07

Abstract

The neutralizing ability of the mycotoxin zearalenone with the KPM-2 biological preparation was studied. Changes in the cytotoxic properties and biochemical parameters of cell cultures under the influence of zearalenone on the background of the use of the drug KPM-2 were studied. As a result of the conducted studies, it is clear that the cytotoxicity of zearalenone in the minimum dose did not differ from the control and at the maximum dose of 3*10^-3 M, cell death was higher by 75% in comparison with the control. When using the drug KPM-2 in a concentration of 2*10^-9 CFU/ml, the cytotoxicity of zearalenone decreased only by 44.9% in comparison with the control. When zearalenone was exposed to cell culture at a dose of 3*10^-3 M, the activity of the enzyme alanine aminotransferase (ALT) increased by 51.7% compared to the control group, and when using the drug KPM-2 at a concentration of 2*10^-9 CFU/ml, ALT activity was higher than control by only 32.1%, respectively. The activity of aspartate aminotransferase (AST) in the group with a concentration of zearalenone 3*10^-3 M increased by 53.9% compared to the control group, when using the drug KPM-2, the neutralization of the toxic effect of zearalenone was observed and the AST index increased by only 44.0%. The concentration of lactic acid when exposed to zearalenone at a dose of 3 * 10^-3 M decreased by 26.1%, and in the group after neutralization of zearalenone with KPM-2 in a concentration of 2*10^-9 CFU/ml, the decrease in this indicator was only 16% compared to the control. The concentration of lactic acid when exposed to zearalenone at a dose of 3 * 10^-3 M decreased by 26.1%, and in the group after neutralization of zearalenone with KPM-2 in a concentration of 2*10^-9 CFU/ml, the decrease in this indicator was only 16% compared to the control. It is proposed to use the drug KPM-2 in the form of a suspension, which has the ability to neutralize the mycotoxin zearalenone and reduce the toxic effect on living organizations.

References

[1] Anon 1988. Understanding and controlling mycotoxins. Anon. Turkey World, 64(4): 12-14.
[2] Antipov, V.A., Vasiliev, V.F., Kutischeva, T.G. 2007. Mycotoxicoses - an important problem of animal husbandry. Veterinary Medicine, 11: 7-9.
[3] Bezborodova, N.A. 2009. Monitoring of mycotoxins in feed and feed raw materials and clinical and immunological features of animal mycotoxicoses in the Ural region: abstract. Diss. Cand. vet. sc. Yekaterinburg, 20 p.
[4] Chen, K.H., et al. 2014. Role of mitofusine 2 (Mfn2) in controlling cellular proliferation. FASEB J., 28: 382–394.
[5] Chulkov, D. C. 2007. Prevention of mycotoxins of animals. Veterinary science, 12: 8-10.
[6] Danicke, S. 2004. Prevention and control of mycotoxins in the poultry production chain: a European view. S. Danicke. Worlds Poultry Science Journal, 58(4): 451-474.
[7] Diakonov, L.P. 2009. Animal cell in culture (Methods and application in biotechnology). M.: Sputnik
[8] Diaz, D. 2006. Mycotoxins and mycotoxicoses. M.: Pechatny Gorod, 376 p.
[9] Dominov, R.R. 2003. Application of the enterosorbent "POLYSORB VP" in poultry farming. Bio, 4: 7-9.
[10] Dorozhkin, V.I., and Ivanov, A.V. 2010. Methodological recommendations for the prevention of mycotoxicosis in animals. Moscow, 114 p.
[11] Fink-Gremmels, J. 1999. Mycotoxins their implication for human and animal health. Veterinary Quatery, 21: 115-120.
[12] Fisinin, V.I. 2006. Guidelines for rationing poultry feeding; eds. Fisinina, S.A. Imangulova, I.A. Egorova, T.M. Okolelova / VNITIP. - Sergiev Posad, 65 p. (in Russian)
[13] Freshney, R.J. 2010. Culture of animal cells 5th ed. Alan R. Liss, Inc., New York. 691 p.
[14] Gajecka, M. 2007. Histopathological and immunohistochemical examinations, and changes in proliferation activity of the uterus in bitches following zearalenon micotoxicosis. Pol. J Vet Sci., 10(3): 143-51.
[15] Gareis, M. et al. 1989. Contamination of cereals and feed with Fusarium mycotoxins in European countries. Fusarium mycotoxins. Path ”Seminar in Warsaw, Sept., 1987”, 540-543.
[16] Grenier, B., and Applegate, T.J. 2013. Modulation of Intestinal Function Following Mycotoxin Ingestion: Meta-Analysis of Published Experiments in Animals. Toxins, 5(2): 396-430.
[17] Idiatov I.I. et al. 2017. Cytotoxic activity of T-2 toxin for a cattle of immortalized fetal lung epithelium cells. Genes and Cells, 12(1): 41-46.
[18] Krska, R. et al. 2012. Guide to Mycotoxins featuring Mycotoxin Risk Management in Animal Production. BIOMIN edition.
[19] Lemmens, M. 2016. Mycotoxin Summer Academy – Module 1. IFA Tulln.
[20] Mabbett, T. 1999. Mycotoxin threat. Feeding times, 4(3): 4-6.
[21] Marin, S. et al. 2013. Mycotoxins: Occurrence, toxicology, and exposure assessment. Food and Chemical Toxicology, 60: 218-237.
[22] Martinelli, J.A., Federizzi, L.C. and Benedetti, A.C. 1994. Redução no rendimento de grãos de aveia em função da severidade da ferrugem da folha. p.116-118.
[23] McKenzie, K.S. et al. 1999. Assessment of the estrogenic effects of zearalenone after treatment with ozone utilizing the mouse uterine weight bioassay. J Toxicol Environ Health A., 56(4): 283-95.
[24] Moule, Y. 1985. Biochemical effects of mycotoxins. Mycotoxins - production, isolation, separation and purification. Toxicology Letters, 6: 37-44.
[25] Muschen, H. and Frank, K.1994. Mycotoxins in oilseeds and risks in animal production. Moulds, Mycotoxins, and Food Preservatives in the Food Industry. Parsippany, New Jersey, BASF Corporation, p. 31-35.
[26] Nabatov, A.A., Raginov I.S. 2015. The DC-SIGN-CD56 interaction inhibits the anti-dendritic cell cytotoxicity of CD56 expressing cells. J Infectious Agents and Cancer, 10. DOI: 10.1186/s13027-015-0043-8
[27] Papazyan, T.T. 2002. Mycotoxines: economic risk and control. Animal husbandry of Russia, 7: 16-18.
[28] Semenov E.I. et al. 2018. Systematic anaphylaxis due to combined mycotoxicosis in wister rats. Indian Veterinary Journal, 95(6): 16-19.
[29] Tiemann, U. et al. 2003. Effects of the mycotoxins α- and β-zearalenol on regulation of progesterone synthesis in cultured cells from porcine ovaries. Reproductive Toxicology, 17: 673 – 81.
[30] Tkachev, A.V., and Tkacheva, O.L. 2017. Comparison of the cytotoxic effects of zearalenon and t-2 toxin on the horses and oxen germ cell in vitro before and after cryopreservation. Tsitologiia, 59(1): 45-52.
[31] Towers, N.R., Sporsen, M., Webber, W. 1995. Zearalenone metabolites in cycling and non-cycling cows. In: Toxinology and Food Safety Research Report, (L.L. Garthwaite, ed). Ruakura Research Center, Hamilton, New Zealand, P. 46-47.
[32] Tremasov, M.Ya. 2012. About the causes of mass mycotoxicosis of animals. Materials of International Mycological Forum, 1: 192-193.
[33] Valiullin L.R. et al. 2018. Study of changes in biochemical parameters of cell cultures when exposed to t-2 toxin. Scientific notes of the Kazan State Academy of Veterinary Medicine n.a. N.E. Bauman, 236(4): 39-43.
[34] Valiullin, L.R. et al. 2014. Reducing the risks of contamination of food raw materials with fungi of the aspergillus genus and their metabolites. Advances in Medical Mycology, 13: 355-356.
[35] Zinedine, A. et al. 2007. Review on the toxicity, occurrence, metabolism, detoxification, regulations and intake of zearalenone: An estrogenic mycotoxin. Food and Chemical Toxicology, 25: 1-18.