Reducing the Risks of Environmental Pollution by Agents of Biological Origin

  • L. R. VALIULLIN Federal Center for Toxicological, Radiation and Biological Safety All Russian Research Institute of Phytopathology, Russian Federation
  • R. S. MUKHAMMADIEV Federal Center for Toxicological, Radiation and Biological Safety, Russian Federation
  • A. S. SOLOVYOVA Federal Center for Toxicological, Radiation and Biological Safety, Russian Federation
  • E. V. SKVORTSOV Federal Center for Toxicological, Radiation and Biological Safety, Russian Federation
  • Rin. S. MUKHAMMADIEV Federal Center for Toxicological, Radiation and Biological Safety, Russian Federation
  • D. A. VALIULLINA N. E. Bauman Kazan State Academy of Veterinary Medicine, Russian Federation
  • N. R. KASANOVA N. E. Bauman Kazan State Academy of Veterinary Medicine, Кazan, Russian Federation

Abstract

The neutralizing ability of the mycotoxin zearalenone with the KPM-2 biological preparation was studied. Changes in the cytotoxic properties and biochemical parameters of cell cultures under the influence of zearalenone on the background of the use of the drug KPM-2 were studied. As a result of the conducted studies, it is clear that the cytotoxicity of zearalenone in the minimum dose did not differ from the control and at the maximum dose of 3*10-3 M, cell death was higher by 75% in comparison with the control. When using the drug KPM-2 in a concentration of 2*10-9 CFU/ml, the cytotoxicity of zearalenone decreased only by 44.9% in comparison with the control. When zearalenone was exposed to cell culture at a dose of 3*10-3 M, the activity of the enzyme alanine aminotransferase (ALT) increased by 51.7% compared to the control group, and when using the drug KPM-2 at a concentration of 2*10-9 CFU/ml, ALT activity was higher than control by only 32.1%, respectively. The activity of aspartate aminotransferase (AST) in the group with a concentration of zearalenone 3*10-3 M increased by 53.9% compared to the control group, when using the drug KPM-2, the neutralization of the toxic effect of zearalenone was observed and the AST index increased by only 44.0%. The concentration of lactic acid when exposed to zearalenone at a dose of 3 * 10-3 M decreased by 26.1%, and in the group after neutralization of zearalenone with KPM-2 in a concentration of 2*10-9 CFU/ml, the decrease in this indicator was only 16% compared to the control. The concentration of lactic acid when exposed to zearalenone at a dose of 3 * 10-3 M decreased by 26.1%, and in the group after neutralization of zearalenone with KPM-2 in a concentration of 2*10-9 CFU/ml, the decrease in this indicator was only 16% compared to the control. It is proposed to use the drug KPM-2 in the form of a suspension, which has the ability to neutralize the mycotoxin zearalenone and reduce the toxic effect on living organizations.

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Published
2020-06-12
How to Cite
VALIULLIN, L. R. et al. Reducing the Risks of Environmental Pollution by Agents of Biological Origin. Journal of Environmental Management and Tourism, [S.l.], v. 11, n. 3, p. 555-562, june 2020. ISSN 2068-7729. Available at: <https://journals.aserspublishing.eu/jemt/article/view/5023>. Date accessed: 29 mar. 2024. doi: https://doi.org/10.14505//jemt.v11.3(43).07.